Journal
METHODS
Volume 33, Issue 3, Pages 220-225Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2003.11.017
Keywords
microscope; GFP; microtubles; digital imaging; time-lapse; fluorescence; confocal
Funding
- NIGMS NIH HHS [R01 GM056836] Funding Source: Medline
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The use of green fluorescent protein (GFP) fusions as biosensors for examining protein localization and dynamics has revolutionized cell biology. Here, we describe the methods developed for imaging of GFP-fusions in the fission yeast Schizosaccharomyces pombe using fluorescence microscopy, with a focus on the use of time-lapse imaging to analyze the dynamics of microtubules. We discuss the considerations in fluorescence microscopy, cell preparation. data acquisition, and image analysis appropriate for analysis of living cells. (C) 2003 Elsevier Inc. All rights reserved.
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