Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 379, Issue 5-6, Pages 777-780Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-004-2529-x
Keywords
exchangeable copper; dialysis; Chelex-100; ICP-MS
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A method based on dialysis in the presence of histidine, and subsequent copper adsorption and preconcentration using Chelex-100 resin, has been developed for selective extraction of exchangeable copper from blood plasma. The method was verified by comparative analysis using two different instrumental techniques, ICP-MS and AAS. The results obtained were in excellent agreement. Accurate quantification of the exchangeable copper and the firmly bound copper was achieved. The method was further validated by analysis of a lyophilised human serum certified reference material.
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