Efficient isolation of polyaromatic fraction from aliphatic compounds in complex extracts using dimethylformamide-pentane partitionings

A liquid-liquid partitioning method was optimized for the rapid and quantitative separation of polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs) from aliphatic hydrocarbons in complex primary extracts. This technique was based on the selective extraction of PAHs and PCBs from an aliphatic solvent into dimethylformamide (DMF). Partition experiments demonstrated that the optimal performance was achieved with a DMF (5% H2O)-n-pentane binary system. The optimized application of two consecutive DMF (5% H2O)-n-pentane treatments to extracts from two different polluted sediments facilitated the elimination of alkanes and unresolved complex mixture by more than 94% while the average recoveries of spiked deuterated-PAHs and C-13 labeled PCBs ranged from 84 to 94 and 75 to 96%, respectively. (C) 2004 Elsevier B.V. All rights reserved.