Journal
MOLECULAR CELL
Volume 15, Issue 1, Pages 31-41Publisher
CELL PRESS
DOI: 10.1016/j.molcel.2004.06.016
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Funding
- NCI NIH HHS [P01-CA92584] Funding Source: Medline
- NIGMS NIH HHS [R01 GM032431, R01-GM32431, R01-GM45190] Funding Source: Medline
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Mismatch-provoked excision directed by a strand break located 3' or 5' to the mispair has been reconstituted using purified human proteins. While MutSalpha, EXOI, and RPA are sufficient to support hydrolysis directed by a 5' strand break, 3' directed excision also requires MutLalpha, PCNA, and RFC. EXOI interacts with PCNA. RFC and PCNA suppress EXOI-mediated 5' to 3' hydrolysis when the nick that directs excision is located 3' to the mispair and activate 3' to 5' excision, which is dependent on loaded PCNA and apparently mediated by a cryptic EXOI 3' to 5' hydrolytic function. By contrast, RFC and PCNA have only a limited effect on 5' to 3' excision directed by a 5' strand break.
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