Journal
APPLIED SURFACE SCIENCE
Volume 257, Issue 24, Pages 10795-10801Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.apsusc.2011.07.102
Keywords
Electrografting; Zwitterions; XPS; ATR-FTIR; CLSM; Contact angle; Biofouling; 2-(Dimethylamino)ethyl methacrylate; Sulfobetaine
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Funding
- Slovak Research and Development Agency [RPEU-0007-06]
- Scientific Grant Agency of the Ministry of Education of Slovak Republic [2/0152/10]
- EU, P. Cezanne [IP-031867]
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The sulfobetaine films were prepared on stainless steel and golden surfaces. In the first step, the poly(2-dimethylamino) ethyl methacrylate) film was created by employing the electrografting polymerization technique. In the second step, this film was modified to polysulfobetaine, i.e. the polymer film bearing the zwitterionic groups. The presence of the electrografted film and its modification were determined by contact angle measurements, infrared spectroscopy in reflectance mode and X-ray photoelectron spectroscopy. The prepared films were homogeneous with the thickness from about 5 to 26 nm as determined by X-ray photoelectron spectroscopy. The atomic force microscopy measurements showed the increase of surface roughness upon the surface coating. In vitro tests using adherent RAT-2 fibroblast cells and fluorescently labelled bovine serum albumin proteins showed that prepared polysulfobetaine films can be used in applications requiring the resistance against cell attachment and biofouling. (C) 2011 Elsevier B.V. All rights reserved.
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