Journal
ANALYTICA CHIMICA ACTA
Volume 515, Issue 2, Pages 255-260Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2004.03.008
Keywords
fluorescent probe; 1-(2,3-Epoxypropoxy)-2-phenylazo-8-acetylaminonaphthalene-3,6-disulfonic acid sodium salt; histidine labeling
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A novel water-soluble fluorescent probe, 1-(2,3-epoxypropoxy)-2-phenylazo-8-acetylamino-naphthalene-3,6-disulfonic acid sodium salt (EPANS), was designed and synthesized by reacting epichlorohydrin with azophloxine, and its properties for labeling of histidine were studied. In alkaline media, EPANS reacted selectively with histidine rather than with other amino acids, causing a large decrease in fluorescence intensity and thereby allowing a selective detection of histidine without a tedious separation. The linear range was 4-500 muM with a detection limit of 1 muM (S/N = 3). The proposed method has been successfully used to determine histidine in human serum. The obtained results were in good agreement with those given by using histidine-nickel complex adsorptive voltammetry. (C) 2004 Elsevier B.V. All rights reserved.
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