Journal
JOURNAL OF NEUROSCIENCE
Volume 24, Issue 28, Pages 6410-6415Publisher
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.1421-04.2004
Keywords
capsaicin; hypersensitivity; nociception; sensory neurons; skin; pain
Categories
Funding
- NINDS NIH HHS [NS31826, R01 NS031826, R01 NS044094, NS33730, NS044094, R01 NS033730, R01 NS023725, NS23725, R56 NS023725] Funding Source: Medline
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Vanilloid receptor 1 (TRPV1) has been proposed to be the principal heat-responsive channel for nociceptive neurons. The skin of both rat and mouse receives major projections from primary sensory afferents that bind the plant lectin isolectin B4 (IB4). The majority of IB4-positive neurons are known to be heat-responsive nociceptors. Previous studies suggested that, unlike rat, mouse IB4-positive cutaneous afferents did not express TRPV1 immunoreactivity. Here, multiple antisera were used to confirm that mouse and rat have different distributions of TRPV1 and that TRPV1 immunoreactivity is absent in heat-sensitive nociceptors. Intracellular recording in TRPV1(-/-) mice was then used to confirm that TRPV1 was not required for detecting noxious heat. TRPV1(-/-) mice had more heat-sensitive neurons, and these neurons had normal temperature thresholds and response properties. Moreover, in TRPV1(-/-) mice, 82% of heat-responsive neurons did not express immunoreactivity for TRPV2, another putative noxious heat channel.
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