Mechanisms involved in the antiplatelet activity of rutin, a glycoside of the flavonol quercetin, in human platelets

The aim of this study was to systematically examine the inhibitory mechanisms of rutin, a well-known flavonoid in platelet aggregation. In this study, rutin concentration-dependently (250 and 290 muM) inhibited platelet aggregation in human platelets stimulated by agonists (i.e., collagen). Rutin (250 and 290 muM) did not significantly interfere with the binding of FITC-triflavin to the glycoprotein IIb/ Ilia complex in human platelets. Rutin (250 and 290 muM) markedly inhibited intracellular Ca2+ mobilization and thromboxane A(2) formation in human platelets stimulated by collagen. Rapid phosphorylation of a platelet protein of M-r 47000 (P47), a marker of protein kinase C activation, was triggered by collagen (1 mug/mL). This phosphorylation was markedly inhibited by rutin (250 and 290 muM). On the other hand, rutin (250 and 290 muM) did not significantly increase the formations of cyclic AMP and nitric oxide/cyclic GMP in platelets. In conclusion, these results indicate that the antiplatelet activity of rutin may involve the following pathways: rutin inhibited the activation of phospholipase C, followed by inhibition of protein kinase C activity and thromboxane A(2) formation, thereby leading to inhibition of the phosphorylation of P47 and intracellular Ca2+ mobilization, finally resulting in inhibition of platelet aggregation.