Journal
FEMS MICROBIOLOGY LETTERS
Volume 236, Issue 2, Pages 341-347Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.femsle.2004.06.010
Keywords
Mycobacterium tuberculosis; two-component system; response regulator; histidine kinase; intracellular; macrophage
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Funding
- NIAID NIH HHS [R01 AI046428, AI46428] Funding Source: Medline
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In the Mycobacterium tuberculosis H37Rv genome, there are I I paired two-component regulatory system genes, two orphan histidine kinase genes, and six orphan response regulator genes. Expression of the 17 response regulator genes and the two orphan histidine kinase genes during growth of M. tuberculosis in human peripheral blood monocyte-derived macrophages has been analyzed by using cDNA mixtures prepared by the selective capture of transcribed sequences (SCOTS) technique. SCOTS probes were prepared from cDNA obtained from M. tuberculosis grown for 18, 48, and I 10 It in human macrophages. Based on expression profiles, the regulatory genes were assigned to three categories: (i) constitutively expressed during growth in macrophages (three genes),; (ii) differentially expressed during growth in macrophages (nine genes) and (iii) no detectable expression during growth in macrophages (seven genes). (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
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