Journal
ANALYTICAL BIOCHEMISTRY
Volume 330, Issue 2, Pages 317-331Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2004.04.004
Keywords
electrospray ionization mass spectrometry; lipidome; lipidomics; two-dimensional mass spectrometry
Funding
- NHLBI NIH HHS [P01 HL 57278, R01 HL 41250] Funding Source: Medline
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Cellular lipids are composed of linear combinations of aliphatic chains covalently attached to backbones and/or head groups that collectively represent the cellular lipidome. Herein we present a novel two-dimensional electrospray ionization mass spectrometric approach to fingerprint most of the major and many of the minor lipid classes in the hepatic cellular lipidome, which collectively represent > 80% of the total lipid mass, directly from their chloroform extracts. Through lipid class-selective intrasource ionization and subsequent analysis of two-dimensional cross-peak intensities, the chemical identity and mass composition of individual molecular species of most mouse hepatic lipid classes were determined from its chloroform extract. This new integrated platform provides a robust foundation for the automated analysis of multidimensional mass spectra to advance the level of analytical scripting in lipidomics to a new plateau. (C) 2004 Elsevier Inc. All rights reserved.
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