Journal
JOURNAL DE MYCOLOGIE MEDICALE
Volume 25, Issue 2, Pages 108-115Publisher
MASSON EDITEUR
DOI: 10.1016/j.mycmed.2014.12.004
Keywords
Alkaliphilic actinomycete; Antifungal production; Aspergillus; Optimization; Response surface methodology
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Objective. - Optimization of medium components and physicochemical parameters for antifungal production by an alkaliphilic and salt-tolerant actinomycete designated Streptomyces sp. SY-BS5; isolated from an arid region in south of Algeria. Materials and methods. - The strain showed broad-spectrum activity against pathogenic and toxinogenic fungi. Identification of the actinomycete strain was realized on the basis of 16S rRNA gene sequencing. Antifungal production was optimized following one-factor-at-a-time (OFAT) and response surface methodology (RSM) approaches. The most suitable medium for growth and antifungal production was found using one-factor-at-a-time methodology. The individual and interaction effects of three nutritional variables, carbon source (glucose), nitrogen source (yeast extract) and sodium chloride (NaCl) were optimized by Box-Behnken design. Finally, culture conditions for the antifungal production, pH and temperature were studied and determined. Results. - Analysis of the 16S rRNA gene sequence (1454 nucleotides) assigned this strain to Streptomyces genus with 99% similarity with Streptomyces cyaneofuscatus JCM4364(T), the most closely related. The results of the optimization study show that concentrations 3.476 g/L of glucose, 3.876 g/L of yeast extract and 41.140 g/L of NaCl are responsible for the enhancement of antifungal production by Streptomyces sp. SY-BS5. The preferable culture conditions for antifungal production were pH 10, temperature 30 degrees C for 09 days. Conclusion. - This study proved that RSM is usual and powerful tool for the optimization of antifungal production from actinomycetes. (C) 2015 Elsevier Masson SAS. All rights reserved.
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