Journal
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
Volume 75, Issue 1-2, Pages 51-56Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jphotobiol.2004.05.002
Keywords
photochromism; polarity; fluorescence; live cells; Bi-stable switch
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Funding
- NCRR NIH HHS [RR 11092, RR 14892] Funding Source: Medline
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The spectroscopic properties, ultrafast kinetics and utilization of a photochromic molecule as a bi-stable fluorescing sensor of polarity in live cells are described. This molecule is a photochromic fulgimide, 2,3-dialkylidenesuccinimide, which emits fluorescence that can be switched optically on and off. The fluorescence intensity is a function of the polarity of the molecular environment, namely it fluoresces strongly when the molecule is in its polar isomeric structure form. We demonstrate that this molecule enters live cells without inducing damage, it binds primarily to internal membranous organelles, (mitochondria) and its fluorescence can be switched optically on and off repeatedly while inside the living cell. A possible use as a bi-stable, on/off sensor is discussed. (C) 2004 Published by Elsevier B.V.
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