A fast photoswitch for minimally perturbed peptides:: Investigation of the trans→cis photoisomerization of N-methylthioacetamide

Thio amino acids can be integrated into the backbone of peptides without significantly perturbing their structure. In this contribution we use ultrafast infrared and visible spectroscopy as well as state-of-the-art ab initio computations to investigate the photoisomerization of the trans form of N-methylthioacetamide (NMTAA) as a model conformational photoswitch. Following the S-2 excitation of trans-NMTAA in water, the return of the molecule into the trans ground state and the formation of the cis isomer is observed on a dual time scale, with a fast component of 8-9 ps and a slow time constant of similar to250 ps. On both time scales the probability of isomerization to the cis form is found to be 30-40%, independently of excitation wavelength. Ab initio CASPT2//CASSCF photochemical reaction path calculations indicate that, in vacuo, the trans --> cis isomerization event takes place on the S-1 and/or T-1 triplet potential energy surfaces and is controlled by very small energy barriers, in agreement with the experimentally observed picosecond time scale. Furthermore, the calculations identify one S-2/S-1 and four nearly isoenergetic S-1/S-0 conical intersection decay channels. In line with the observed isomerization probability, only one of the S-1/S-0 conical intersections yields the cis conformation upon S-1 --> S-0 decay. A substantially equivalent excited-state relaxation results from four T-1/S-0 intersystem crossing points.