Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 807, Issue 1, Pages 61-68Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2004.03.029
Keywords
partitioning; aqueous two-phase systems; enzyme purification; thermoseparation; bromelain
Ask authors/readers for more resources
Bromelain is an enzymatic complex obtained from pineapple (Ananas comosus) fruits and stem. Thermoseparation of bromelain by poly(ethylene oxide) (PEO)- poly(propylene oxide) (PPO)- poly(ethylene oxide) (PEO) block copolymers aqueous solutions was studied. Triblock copolymers with different EO percentages and different molecular mass were evaluated. Copolymer solutions at different pH values, buffer concentrations and copolymer concentrations were investigated. It was found that cloud point temperature increases as a function of %EO and decreases with copolymer molecular mass, copolymer concentration and buffer concentration. The results showed that all the studied factors influenced enzyme partition. The best conditions were copolymer with 10% EO and molecular mass of 2000 g/mol, temperature of 25 degreesC, copolymer concentration of 5% (w/w), pH 6.0 and salt concentration of 15 mM. Enzyme activity recovery around 79.5%, purification factor around 1.25 and activity partition coefficient around 1.4 were obtained. (C) 2004 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available