Journal
JOURNAL OF NEUROSCIENCE
Volume 24, Issue 30, Pages 6650-6658Publisher
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.0010-04.2004
Keywords
NGF; tyrosine phosphorylation; basal forebrain; adenosine; PACAP; Golgi apparatus
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Funding
- NICHD NIH HHS [HD23315, P01 HD023315] Funding Source: Medline
- NINDS NIH HHS [R56 NS021072, NS21072, R01 NS021072] Funding Source: Medline
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Neurotrophins, such as NGF and BDNF, activate Trk receptor tyrosine kinases through receptor dimerization at the cell surface followed by autophosphorylation and intracellular signaling. It has been shown that activation of Trk receptor tyrosine kinases can also occur via a G-protein-coupled receptor ( GPCR) mechanism, without involvement of neurotrophins. Two GPCR ligands, adenosine and pituitary adenylate cyclase-activating polypeptide ( PACAP), can activate Trk receptor activity to increase the survival of neural cells through stimulation of Akt activity. To investigate the mechanism of Trk receptor transactivation, we have examined the localization of Trk receptors in PC12 cells and primary neurons after treatment with adenosine agonists and PACAP. In contrast to neurotrophin treatment, Trk receptors were sensitive to transcriptional and translational inhibitors, and they were found predominantly in intracellular locations particularly associated with Golgi membranes. Biotinylation and immunostaining experiments confirm that most of the transactivated Trk receptors are found in intracellular membranes. These results indicate that there are alternative modes of activating Trk receptor tyrosine kinases in the absence of neurotrophin binding at the cell surface and that receptor signaling may occur and persist inside of neuronal cells.
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