Journal
FEBS LETTERS
Volume 571, Issue 1-3, Pages 182-186Publisher
WILEY
DOI: 10.1016/j.febslet.2004.07.002
Keywords
phytoalexin; diterpene cyclase; stemar-13-ene; oryzalexin S; Oryza sativa L.
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In suspension-cultured rice cells, diterpenoid phytoalexins are produced in response to exogenously applied elicitors. We isolated a cDNA encoding a diterpene cyclase, OsDTC2, from suspension-cultured rice cells treated with a chitin elicitor. The OsDTC2 cDNA was overexpressed in Escherichia coli as a fusion protein with glutathione S-transferase, and the recombinant OsDTC2 was indicated to function as stemar-13-ene synthase that converted syn-copalyl diphosphate to stemar-13-ene, a putative diterpene hydrocarbon precursor of the phytoalexin oryzalexin S. The level of OsDTC2 mRNA in suspension-cultured rice cells began to increase 3 h after addition of the elicitor and reached the maximum after 8 h. The expression of OsDTC2 was also induced in UV-irradiated rice leaves. In addition, we indicated that stemar-13-ene accumulated in the chitin-elicited suspension-cultured rice cells and the UV-irradiated rice leaves. (C) 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.
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