Journal
MOLECULAR AND CELLULAR ENDOCRINOLOGY
Volume 222, Issue 1-2, Pages 113-120Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.mce.2004.04.009
Keywords
steroid 5 alpha-reductase; mouse testes; neonatal androgen; androstanediol; dihydrotestosterone
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Funding
- NIDDK NIH HHS [R21DK59942] Funding Source: Medline
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5alpha-Androstane-3alpha, 17beta-diol (androstanediol) is the predominant androgen in immature mouse testes, and studies were designed to investigate its pathway of synthesis, the steroid 5alpha-reductase isoenzyme involved in its formation, and whether testicular androstanediol is formed in embryonic mouse testes at the time of male phenotypic development. In 24-26-day-old immature testes, androstanediol is formed by two pathways; the predominant one involves testosterone --> dihydrotestosterone --> androstanediol, and a second utilizes the pathway progesterone --> 5alphadihydroprogesterone --> 5alpha-pregnane-3alpha-ol-20-one --> 5alpha-pregnane-3alpha, 17alpha-diol-20-one --> androsterone --> androstanediol. Formation of androstanediol was normal in testes from mice deficient in steroid 5alpha-reductase 2 but absent in testes from mice deficient in steroid 5alpha-reductase 1, indicating that isoenzyme 2 is not expressed in day 24-26 testes. The fact that androstenedione and testosterone were the only androgens identified after incubation of day 16 and 17 embryonic testes with [H-3]progesterone implies that androstanediol formation in the testis plays no role in male phenotypic differentiation in the mouse. (C) 2004 Elsevier Ireland Ltd. All rights reserved.
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