Journal
FEBS LETTERS
Volume 571, Issue 1-3, Pages 99-102Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2004.06.067
Keywords
cell adhesion; sialic acid; signal transduction; calcium; galectin-3
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Sialylation of glycoconjugates is essential for mammalian cells. Sialic acid is synthesized in the cytosol from N-acetylmannosamine by several consecutive steps. Using N-propanoylmannosamine, a novel precursor of sialic acid, we are able to incorporate unnatural sialic acids with a prolonged N-acyl side chain (e.g., N-propanoylneuraminic acid) into glycoconjugates taking advance of the cellular sialylation machinery. Here, we report that unnatural sialylation of HL60-cells leads to an increased release of intracellular calcium after application of thapsigargin, an inhibitor of SERCA Ca2+-ATPases. Furthermore, this increased intracellular calcium concentration leads to an increased adhesion to fibronectin. Finally, we observed an increase of the lectin galectin-3, a marker of monocytic differentiation of HL60-cells. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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