Journal
BIOSENSORS & BIOELECTRONICS
Volume 20, Issue 1, Pages 3-8Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2003.11.027
Keywords
amperometric detection; electrochemical sensor; artificial peroxidase; morphine; Prussian blue
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In this work, the electrocatalytic oxidation of morphine (MO) at an optically transparent indiurn tin oxide (ITO) electrode modified by an electrodeposited Prussian blue (PB) thin film is first demonstrated, and the amperometric detection of MO was then investigated. Experimental results showed that the thin film on the ITO surface, confined to the PB/Berlin green (BG) redox pair, can serve as an excellent mediator which facilitates electron transfer and considerably lowers the overpotential required, as compared to a bare ITO electrode. Thus, PB can be regarded as a promising artificial peroxidase for MO. The rate of such an electrocatalytic reaction is pH dependent with the highest value at pH 5. By potential-step excitation from 0.55 to 0.70 V, a linear calibration curve, displaying the relationship between steady-state currents and MO concentrations (ranging from 0.09 to 1.0 mM), was obtained. The detection sensitivity is about 16.8 muA/cm(2) mM. Most importantly, the method described herein can readily discriminate MO analogs lacking the phenolic -OH group, such as codeine, and can thus benefit the specific recognition of MO. (C) 2004 Elsevier B.V. All rights reserved.
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