Journal
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
Volume 70A, Issue 2, Pages 159-168Publisher
WILEY
DOI: 10.1002/jbm.a.30053
Keywords
themoresponsive polymer; poly(N-isopropyl acrylamide); cell adhesion; microheater array; cell patterning; biochip
Funding
- NCRR NIH HHS [RR-01296] Funding Source: Medline
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A novel approach is reported for cell patterning based on addressable microheaters and a poly(N-isopropyl acrylamide) (pNIPAM) themoresponsive coating. This thermoresponsive coating is created by a radio frequency NIPAM plasma and is denoted as plasma polymerized NIPAM (ppNIPAM). Films of ppNIPAM with a good retention of monomer side-chain functionality are produced using low-power continuous plasma deposition. Cell adhesion and cell detachment tests indicate that the surface switches between adhesive and nonadhesive behaviors as a function of temperature. The use of a photolithographically fabricated microheater array allows the ppNIPAM transition to occur spatially under the control of individual heaters. This localized change in the surface adhesive behavior is used to direct site-specific cell attachment. Patterned adhesion of two types of cells has been visualized on the array through fluorescent markers. Applications for diagnostic devices, cell-based sensors, tissue engineering, and cell transfection are envisioned. (C) 2004 Wiley Periodicals, Inc.
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