Journal
JOURNAL OF BACTERIOLOGY
Volume 186, Issue 15, Pages 5172-5177Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/jb.186.15.5172-5177.2004
Keywords
-
Categories
Ask authors/readers for more resources
The six copies of the response regulator CheY from Rhodobacter sphaeroides bind to the switch protein FliM. Phosphorylation by acetyl phosphate (AcP) was detected by tryptophan fluorescence quenching in three of the four CheYs that contain this residue. Autophosphorylation with (AcP)-P-32 was observed in five CheY proteins. We also show that all of the cheY genes are expressed simultaneously; therefore, in vivo all of the CheY proteins could bind to FliM to control the chemotactic response. Consequently, we hypothesize that in this complex chemotactic system, the binding of some CheY proteins to FliM, does not necessarily imply switching of the flagellar motor.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available