Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 70, Issue 8, Pages 5019-5025Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.70.8.5019-5025.2004
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We report an easy procedure for isolating chromosome-clustered genes. By following this methodology, the entire set of genes belonging to the phenylacetic acid (PhAc; 18-kb) pathway as well as those required for the synthesis and mobilization of different polyhydroxyalkanoates (PHAs; 6.4 kb) in Pseudomonas putida U were recovered directly from the bacterial chromosome and cloned into a plasmid for the first time. The transformation of different bacteria with these genetic constructions conferred on them the ability to either degrade PhAc or synthesize bioplastics (PHAs).
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