Journal
CHEMICAL ENGINEERING JOURNAL
Volume 101, Issue 1-3, Pages 151-156Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.cej.2003.11.016
Keywords
flow-through PCR; microfabrication; PDMS
Categories
Ask authors/readers for more resources
This study presents a microfabricated device for polymerase chain reaction (PCR) with a flow-through manner for use in environmental microbiology. The device was developed utilizing photolithography and softlithography techniques and evaluated as one component of a totally integrated in situ gene analysis system. The developed device was composed of a glass-based temperature control chip and polydimethylsiloxane (PDMS)-based microchannel chip. For the temperature control chip, six heaters made from indium tin oxide (ITO) are placed on a glass substrate to define three uniform temperature zones for flow-through PCR. On the each heater, a platinum (Pt) line was placed as a temperature sensor. The PDMS microchannel structure was fabricated by using a molding method with a negatively patterned mold master. The width and depth of folded microchannel was 100 mum and total length for 30 cycles of flow-through PCR was approximately 3.0 m. With the flow-through PCR device, 580 and 1450 bp (base pairs) of DNA fragments were successfully amplified from Escherichia coli genomic DNA and directly from untreated cells. (C) 2003 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available