Journal
JOURNAL OF CLINICAL MICROBIOLOGY
Volume 42, Issue 8, Pages 3649-3654Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/jcm.42.8.3649-3654.2004
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Members of the genus Brucella are categorized as biothreat agents and pose a hazard for both humans and animals. Current identification methods rely on biochemical tests that may require up to 7 days for results. We sequenced the 16S rRNA genes of 65 Brucella strains along with 17 related strains likely to present a differential diagnostic challenge. All Brucella 16S rRNA gene sequences were determined to be identical and were clearly different from the 17 related strains, suggesting that 16S rRNA gene sequencing is a reliable tool for rapid genus-level identification of Brucella spp. and their differentiation from closely related organisms.
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