Sequential extraction of copper from soils and relationships with copper in maize

We studied copper uptake by maize grown on soils that have been contaminated with CuSO4, In soil the total copper level ranged from 24 to 135 mg kg(-1). The copper distribution in soil fractions was assessed by sequential extraction, showing that anthropogenic copper is mainly concentrated in oxides fractions. The copper concentration of maize at the maturity stage reached values from 36.3 to 65.9 mg kg(-1) compared to copper levels usually found in non-contammated crops (5-30 mg kg(-1)). Here we demonstrate that copper can be accumulated by maize and that copper concentration in maize can be predicted by equations including copper concentration of soil fractions.