Compositions of anthocyanin and other flavonoids in cultured cells of rabbiteye blueberry (Vaccinium ashei Reade cv. Tifblue)

Our blueberry cultured cells produce anthocyanins in high quantity (Nawa et al., Biosci. Biotech. Biochem., 57, 770-774, 1993). Using high performance liquid chromatography/mass spectrometry (LC-MS), 14 anthocyanins in the red cells derived from leaves of rabbiteye blueberry cv. Tifblue (Vaccinium ashei Reade) were identified as 3-galactoside (gal), 3-glucoside (glc) and 3-arabinoside (ara) of cyanidin (Cy), delphinidin (Dp), petunidin (Pe), peonidin (Pn) and malvidin (Mv), except for My 3-ara. The fruits additionally contained 15 anthocyanins and My 3-ara, as a main anthocyanin. The autumn red leaves contained Cy 3-gal, Cy 3-ara and Cy 3-glc. In the red cells and the leaves, derivatives of Cy were the most abundant at 85.7% (Cy 3-gal 49.1%, Cy 3-glc 5.8%, Cy 3-ara 30.9%) and 100% (Cy 3-gal 51.0%, Cy 3-glc 6.5%, Cy 3-ara 42.5%) of the total anthocyanins, respectively. In contrast, the fruits contained My derivatives as main components that amounted to 50.9% of the total anthocyanins; My 3-gal, My 3-ara and My 3-glc constituted 21.2, 15.3 and 14.4%, respectively. Cy derivatives in fruit anthocyanins amounted to 18.0%. The red cells produced 107 mg of anthocyanin/100 g fr wt, which was slightly lower than that of the fruits (129 mg). LC-MS analysis also suggested the presence of quercetin (Q) 3-glc, Q 3-rutinoside, and glycosides of myricetin (M) and Q in the red cells. This analysis showed the presence of (+)-catechin (C) and (-)-epicatechin (EC) in these cells as well as of (+)- C, (-)-EC and chlorogenic acid in the red leaves. These flavonoids and phenolics were also some of the main components in the leaves.