Journal
BIOMATERIALS
Volume 25, Issue 17, Pages 3583-3592Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2003.10.033
Keywords
patterned co-cultures; layer-by-layer; hyaluronic acid; poly-L-lysine
Funding
- NHLBI NIH HHS [HL60435] Funding Source: Medline
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A novel method for patterning cellular co-cultures that uses the layer-by-layer deposition of ionic biopolymers is described. Non-biofouling hyaluronic acid (HA) micropatterns were used to immobilize cells and proteins to glass substrates. Subsequent ionic adsorption of poly-L-lysine (PLL) to HA patterns was used to switch the HA surfaces from cell repulsive to adherent thereby facilitating the adhesion of a second cell type. The utility of this approach to pattern co-cultures of hepatocytes or embryonic stem cells with fibroblasts was demonstrated. In addition, the versatility of this approach to generate patterned co-cultures irrespective of the primary cell seeding and relative adhesion of the seeded cells was demonstrated. Thus, the proposed method may be a useful tool for fabricating controlled cellular co-cultures for cell-cell interaction studies and tissue engineering applications. (C) 2003 Elsevier Ltd. All rights reserved.
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