Journal
PLANT PHYSIOLOGY
Volume 135, Issue 4, Pages 1903-1907Publisher
AMER SOC PLANT BIOLOGISTS
DOI: 10.1104/pp.104.039537
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Isoprene emitted from plants is made in chloroplasts from dimethylallyl pyrophosphate (DMAPP). Leaves of Populus nigra and Phragmites australis exposed to (CO2)-C-13 for 15 min emitted isoprene that was about 90% C-13, but DMAPP isolated from those leaves was only 28% and 36% C-13, respectively. The labeled DMAPP is likely to represent chloroplastic DMAPP contributing to isoprene emission. A substantial C-13 labeling was also found in both emission and DMAPP pool of low-emitting, young leaves of Phragmites. This confirms that low emission of young leaves is not caused by absence of chloroplastic DMAPP but rather by enzyme characteristics. A very low C-13 labeling was found in the DMAPP pool and in the residual isoprene emission of leaves previously fed with fosmidomycin to inhibit isoprene formation. This shows that fosmidomycin is a very effective inhibitor of the chloroplastic biosynthetic pathway of isoprene synthesis, that the residual isoprene is formed from extra-chloroplastic sources, and that chloroplastic and extrachloroplastic pathways are not cross-linked, at least following inhibition of the chloroplastic pathway Refixation of unlabeled respiratory CO, in the light may explain incomplete labeling of isoprene emission, as we found a good association between these two parameters.
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