Journal
JOURNAL OF PHYCOLOGY
Volume 40, Issue 4, Pages 705-710Publisher
BLACKWELL PUBLISHING INC
DOI: 10.1111/j.1529-8817.2004.03226.x
Keywords
autofluorescence; flow cytometry; Pseudo-nitzschia
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A flow cytometer coupled to a scanning monochromator and a fluorescence microscope were used to characterize the fluorescence spectrum of Pseudo-nitzschia multiseries (Haste) Haste, a pennate diatom that produces the neurotoxin domoic acid, a lethal amnesic. In this research, we characterize the fluorescence spectrum of P multiseries in vivo over the wavelength range of 360 to 850 nm and show that this diatom autofluoresces blue when excited with UV light (350-365 nm). The autofluorescence characterization of Pseudo-nitzschia may provide new methods for rapid in situ monitoring of diatom populations and reiterates the usefulness of flow cytometry in the analysis and study of marine phytoplankton.
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