Journal
BRAIN RESEARCH PROTOCOLS
Volume 13, Issue 3, Pages 183-188Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.brainresprot.2004.05.002
Keywords
corticospinal neuron; fluorescence; Fluoro-Green; Fluoro-Red; retrograde labeling; slice patch clump; rat
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Funding
- NINDS NIH HHS [R01 NS036260-04A2] Funding Source: Medline
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For pathophysiological studies, it is advantageous to label specific neuronal populations in living animals. This study aimed to establish a method for stable and long-lasting fluorescent labeling of corticospinal neurons in the living animal. The two fluorescent dyes Fluoro-Red and Fluoro-Green were injected in the cervical spinal cord of anesthetized newborn rats. After a recovery period, treated rats were returned to the mother. After 24 h and 14 days, fixed brain sections revealed wide-spread fluorescence in elongated or pyramidal-shaped cell profiles in a discrete internal cortical layer, consistent with layer V pyramidal cells. Labeled neurons displayed spontaneous synaptic activity using the slice patch clamp method. These results suggest that these dyes are effective tools for pathophysiological and slice patch clamp studies focused on specific neuron groups. (C) 2004 Elsevier B.V. All rights reserved.
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