Journal
POLYCYCLIC AROMATIC COMPOUNDS
Volume 24, Issue 4-5, Pages 333-342Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/10406630490468478
Keywords
bile; fish; fixed fluorescence; high-performance liquid chromatography method comparison; polycyclic aromatic hydrocarbon metabolite
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Fish exposed to polycyclic aromatic hydrocarbons (PAHs) accumulate only a small amount of PAH in their tissues. Hydrophobic PAHs are readily transformed into hydrophilic metabolites and excreted in bile. Bile samples of five fish species were collected from the Baltic Sea and analyzed using two methods: high-performance liquid chromatography (HPLC) with fluorescence detection and a fixed wavelength fluorescence method. With the HPLC method PAH compounds were quantitated after hydrolysis, and with the fluorescence method without enzyme-assisted hydrolysis. In HPLC analysis the major metabolite in all fish species, 1-hydroxypyrene, was the only metabolite detected in perch and salmon samples. 1-Hydroxyphenanthrene was detected in a few flounder and in most eelpouts. The correlation between the two methods for pyrene-type metabolites was good (r(2) = 0.773).
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