Journal
ENDOCRINOLOGY
Volume 145, Issue 8, Pages 3840-3849Publisher
ENDOCRINE SOC
DOI: 10.1210/en.2003-1512
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- NIDDK NIH HHS [DK-41230] Funding Source: Medline
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Myoblasts transfected with HB10D insulin secrete more hormone than those transfected with wild-type insulin, as published previously, indicating that production of wild-type insulin is not efficient in these cells. The ability of non-beta-cells to produce insulin was examined in several cell lines. In clones of neuroendocrine GH(4)C(1) cells stably transfected with proinsulin, two thirds of S-35-proinsulin was degraded within 3 h of synthesis, whereas S-35-prolactin was stable. In transiently transfected neuroendocrine AtT20 cells, half of S-35-proinsulin was degraded within 3 h after synthesis, whereas S-35-GH was stable. In transiently transfected fibroblast COS cells, S-35-proinsulin was stable for longer, but less than 10% was secreted 8 h after synthesis. Proinsulin formed a concentrated patch detected by immunofluorescence in transfected cells that did not colocalize with calreticulin or BiP, markers for the endoplasmic reticulum, but did colocalize with membrin, a marker for the cis-medial Golgi complex. Proinsulin formed a Lubrol-insoluble aggregate within 30 min after synthesis in non-beta-cells but not in INS-1E cells, a beta-cell line that normally produces insulin. More than 45% of S-35-HB10D proinsulin was secreted from COS cells 3 h after synthesis, and this mutant formed less Lubrol-insoluble aggregate in the cells than did wild-type hormone. These results indicate that proinsulin production from these non-beta-cells is not efficient and that proinsulin aggregates in their secretory pathways. Factors in the environment of the secretory pathway of beta-cells may prevent aggregation of proinsulin to allow efficient production.
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