Journal
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
Volume 14, Issue 15, Pages 4001-4004Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmcl.2004.05.042
Keywords
SELEX; aptamer; cell adhesion; fibronectin; RGD
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An affinity column immunobilizing a decapeptide H2N-RGDSPASSKP-CO2H was used to select RGD-binding aptamers from a pool of 86-mer single-strand oligodeoxynucleotides (ODNs) containing a random 40-mer sequence. The enriched library thus obtained was further selected against adsorbed fibronectin and individual aptamers were monocloned in E coli and sequenced to give a couple of highly homologous ODNs, which indeed inhibited fibronectin-integrin mediated cell adhesion. (C) 2004 Elsevier Ltd. All right, reserved.
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