Journal
BIOPOLYMERS
Volume 74, Issue 5, Pages 345-351Publisher
WILEY
DOI: 10.1002/bip.20080
Keywords
DNA; carvacrol; thymol; binding constant; conformation; FTIR; UV-visible spectroscopy
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Thymol and carvacrol can bind to major and minor grooves of B-DNA. The aim of this study was to examine the interaction of calf thymus DNA with thymol and carvacrol in aqueous solution and physiological pH with thymol/DNA and carvacrol/DNA (phosphate) molar ratios of 1/20, 1/10, 1/5, and 1/1. Fourier transform infrared and UV-visible difference spectroscopy were used to determine the thymol and carvacrol binding mode, binding constant, sequence selectivity, DNA secondary structure, and structural variations of thymol/DNA and carvacrol/DNA complexes in aqueous solution. Spectroscopic evidence showed that the thymol and carvacrol interaction occurred mainly through H-bonding of the thymol and carvacrol OH group to the guanine N7, cytosine N3, and backbone phosphate group with overall binding constant of K(thymol-DNA) = 2.43 X 10(3)M(-1), K(carvacrol-DNA) = 1.55 X 10(3)M(-1). In thymol and carvacrol-DNA complexes, DNA remains in the B-family structure. (C) 2004 Wiley Periodicals, Inc.
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