Overexpressed GM1 suppresses nerve growth factor (NGF) signals by modulating the intracellular localization of NGF receptors and membrane fluidity in PC12 cells

Ganglioside GM1 has been considered to have a neurotrophic factor-like activity. To analyze the effects of endogenously generated GM1, the rat pheochromocytoma cell line PC12 was transfected with the GM1/GD1b/ GA1 synthase gene and showed increased expression levels of GM1. To our surprise, GM1(+)-transfectant cells (GM1(+) cells) showed no neurite formation after stimulation with nerve growth factor (NGF). Autophosphorylation of NGF receptor TrkA and activation of ERK1/2 after NGF treatment were scarcely detected in GM1(+) cells. Binding of I-125-NGF to PC12 cells was almost equivalent between GM1(+) cells and controls. However, dimer formation of TrkA upon NGF treatment was markedly suppressed in GM1(+) cells in both cross-linking analysis with Bis(sulfosuccinimidyl) suberate 3 and I-125-NGF binding assay. The sucrose density gradient fractionation of the cell lysate revealed that TrkA primarily located in the lipid raft fraction moved to the non-raft fraction in GM1(+) cells. p75(NTR) and Ras also moved from the raft to non-raft fraction in GM1(+) cells, whereas flotillin and GM1 persistently resided in the lipid raft. TrkA kinase activity was differentially regulated when GM1 was added to the kinase assay system in vitro, suggesting suppressive/enhancing effects of GM1 on NGF signals based on the concentration. Measurement of fluorescence recovery after photobleaching revealed that the membrane fluidity was reduced in GM1(+) cells. These results suggested that overexpressed GM1 suppresses the differentiation signals mediated by NGF/ TrkA by modulating the properties of the lipid raft and the intracellular localization of NGF receptors and relevant signaling molecules.