Journal
CHEMBIOCHEM
Volume 5, Issue 8, Pages 1081-1087Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.200300905
Keywords
dendrimers; DNA transfection; gene delivery; hyperbranched polyamines; polymers
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Polycationic dendrimers are interesting nonviral vectors for in vitro DNA delivery. We describe a simple approach to the synthesis of dendritic polyamines with different molecular weights and adjustable flexibility (degrees of branching; DB). Both parameters influence the transfection efficiency and the cell toxicity of the polymer. Functionalization of hyperbranched polyethylenimine (PEI) by a two-step procedure generated fully branched pseudo-dendrimers (analogues of polypropylenimine (PPI) and polyamidoamine (PAMAM) dendrimers). The DNA transfection efficiencies observed for these polymers depended on the cell line investigated. The highest efficiencies were observed for polymers whose un-functionalized PEI cores had molecular weights in the range M-w=6000-25000 g mol(-1). The cytotoxicity of the dendrimers 1, generally rises with increasing core size. The data collected for NIH/3T3 and COS-7 cells indicate a maximum transfection efficiency at around 60% branching for the PPI analogues, and at a PEI-core molecular weight of M-w=25000 g mol(-1). PAMAM functionalization of PEI (M-w=5000 and 21000 g mol(-1)) leads to polymers with little or no cytotoxity in the cell lines investigated.
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