Journal
DISEASES OF AQUATIC ORGANISMS
Volume 60, Issue 2, Pages 141-148Publisher
INTER-RESEARCH
DOI: 10.3354/dao060141
Keywords
chytrids; Batrachochytrium dendrobatidis; Amphibian declines; real-time PCR; Taqman assay; chytridiomycosis
Categories
Ask authors/readers for more resources
Batrachochytrium dendrobatidis is a major pathogen of frogs worldwide, associated with declines in amphibian populations. Diagnosis of chytridiomycosis to date has largely relied upon histological and immunohistochemical examination of toe clips. This technique is invasive and insensitive particularly at early stages of infection when treatment may be possible. We have developed a real-time PCR Taqman assay that can accurately detect and quantify one zoospore in a diagnostic sample. This assay will assist the early detection of B. dendrobatidis in both captive and wild populations, with a high degree of sensitivity and specificity, thus facilitating treatment and protection of endangered populations, monitoring of pristine environments and preventing further global spread via amphibian trade.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available