Journal
ONCOGENE
Volume 23, Issue 36, Pages 6083-6094Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/sj.onc.1207804
Keywords
IG20; MADD; TRAIL; DISC; caspase-8; apoptosis; cancer
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Recently, we identified (I) under bar nsulinoma -(G) under bar lucagonoma clone 20 (IG20) that can render cells more susceptible to tumor necrosis factor-alpha (TNF-alpha)-induced apoptosis. In addition, it can slow cell proliferation, and enhance drug- and radiation-induced cell death. TNF-related apoptosis-inducing ligand (TRAIL) can selectively induce apoptosis in some cancer cells and render others susceptible to cotreatment with drugs and irradiation, with little or no effect on most normal cells. In this study, we investigated the potential of IG20 to enhance TRAIL-induced apoptosis and found that it can render cells more susceptible to TRAIL treatment through enhanced activation of caspases. Further, we showed that this effect can be suppressed by caspase inhibitors, p35 and CrmA, and a (d) under bar ominant-(n) under bar egative (F) under bar as- (a) under bar ssociated (d) under bar eath (d) under bar omain-containing protein (DN-FADD). Results from colocalization and immunoprecipitation studies showed that IG20 can interact with TRAIL death receptors (DR), DR4 and DR5 and increase recruitment of FADD and caspase-8 into the TRAIL death-inducing signaling complex (DISC). These results indicate that IG20 is a novel protein that can enhance TRAIL-induced apoptosis by facilitating DISC formation.
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