Journal
INTERNATIONAL JOURNAL OF CANCER
Volume 111, Issue 2, Pages 192-197Publisher
WILEY
DOI: 10.1002/ijc.20245
Keywords
cyclooxgenase-2; gamma-linolenic acid; 15S-hvdroxyeicosatrienoic acid; prostaglandin E-2; prostatic cell
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Emerging reports now implicate alterations of arachidonic acid (AA) metabolism with prostate carcinogenesis. To test this hypothesis, androgen-primed benign hyperplastic (BHC) and malignant tumorigenic (MTC) cells derived from the Lobund-Wistar rat model of autochthonous prostate adenocarcinoma were incubated with 14 C-AA. Our data using MTCs revealed enhanced dual metabolism of 14 C-AA via COX to generate increased PGE(2) and via 5-lipoxygenase (LOX) to generate increased SS-HETE in tumorigenic cells. Western blot of MTCs revealed upregulation of COX-2 expression. This paralleled the increased biosynthesis of PGE(2). Since some polyunsaturated fatty acids have been reported to modulate AA metabolism and tumorigenesis, we primed the cells with either gamma-linolenic acid (GLA) or its in vivo metabolite, 15S-HETrE, prior to incubation with AA. Our data revealed suppression of COX-2 expression/PGE(2) biosynthesis. In parallel, priming cells with 15S-HETrE resulted in greater suppression of COX-2 expression/PGE(2) biosynthesis. These findings suggest that 15S-HETrE could function in vivo after dietary intake of GLA to suppress DHT-enhanced prostatic COX-2 expression/PGE(2) biosynthesis and, thus, alleviate tumor growth and progression. (C) 2004 Wiley-Liss, Inc.
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