Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 321, Issue 2, Pages 275-279Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2004.06.139
Keywords
cell lineage specific disruption; transgenic mouse; Cre; LoxP; EGFP; diphtheria toxin; animal model for human disease
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We have established a transgenic mouse line in which foxed neomycin resistant cassette was inserted between the CA G promoter and EGFP. When these transgenic mice were mated with Cre-expressing transgenic animals, the offspring obtained were fluorescent green. We then established a transgenic mouse line in which EGFP in the above construct was replaced by diphtheria toxin A chain (DT). When the latter transgenic mice were mated with mice expressing Cre restricted to germ cells, we obtained healthy but sterile offspring due to a disruption of germ line cells by DT expression. We predict that this strategy will be useful for the construction of new animal models for human diseases, featuring a variety of missing cell lineages produced by disruption with DT. (C) 2004 Elsevier Inc. All rights reserved.
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