Journal
JOURNAL OF CLINICAL MICROBIOLOGY
Volume 42, Issue 9, Pages 4361-4364Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.42.9.4361-4364.2004
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A real-time PCR assay using SYBR Green I for quantification of provirus load in a murine model of AIDS (i.e., LP-BM5 infection) was developed and validated. In this method, data are normalized against the 18S rRNA gene. The method has a dynamic range of 8 logs and a sensitivity of one copy.
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