Journal
MOLECULAR AND CELLULAR BIOLOGY
Volume 24, Issue 17, Pages 7654-7668Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/MCB.24.17.7654-7668.2004
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Funding
- NCI NIH HHS [R01 CA079721, CA079721, R01 CA093614, CA095441, CA93614, R01 CA095441] Funding Source: Medline
- NEI NIH HHS [EY10572, P30 EY010572] Funding Source: Medline
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The p53-MDM2 feedback loop is vital for cell growth control and is subjected to multiple regulations in response to various stress signals. Here we report another regulator of this loop. Using an immunoaffinity method, we purified an MDM2-associated protein complex that contains the ribosomal protein L23. L23 interacted with MDM2, forming a complex independent of the 80S ribosome and polysome. The interaction of L23 with MDM2 was enhanced by treatment with actinomycin D but not by gamma-irradiation, leading to p53 activation. This activation was inhibited by small interfering RNA against L23. Ectopic expression of L23 reduced MDM2-mediated p53 ubiquitination and also induced p53 activity and G(1) arrest in p53-proficient U2OS cells but not in p53-deficient Saos-2 cells. These results reveal that L23 is another regulator of the p53-MDM2 feedback regulation.
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