Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 70, Issue 9, Pages 5685-5687Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.70.9.5685-5687.2004
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Short-chain-length-medium-chain-length polyhydroxyalkanoates were synthesized in Saccharomyces cerevisiae from intermediates of the beta-oxidation cycle by expressing the polyhydroxyalkanoate synthases from Aeromonas caviae and Ralstonia eutropha in the peroxisomes. The quantity of polymer produced was increased by using a mutant of the beta-oxidation-associated multifunctional enzyme with low dehydrogenase activity toward R-3-hydroxybutyryl coenzyme A.
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