Journal
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
Volume 58, Issue 2, Pages 369-383Publisher
ELSEVIER
DOI: 10.1016/j.ejpb.2004.03.034
Keywords
field-flow fractionation; separation; biopharmaceutical application; protein analytics; particle characterization; review
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Field-flow fractionation (FFF) is a family of flexible analytical fractionating techniques which have the advantage that the separation of analytes is achieved, solely through the interaction of the sample with an external, perpendicular physical field, rather than by the interaction with a stationary phase. The rapid progress in pharmaceutical biotechnology goes along with an increasing demand in potent, high-efficient analytical methods. Thus, FFF techniques are gaining increasing attention for their ability to separate and characterize populations of polymers, colloids and particles of up to about 100 mum in size. It is the intention of this review to provide an overview on common FFF techniques, to summarize inherent advantages and limitations and to introduce both established and challenging applications in the (bio)pharmaceutical field. Thereby, asymmetrical flow FFF is addressed predominantly, since it is the most versatile applicable FFF technique. (C) 2004 Elsevier B.V. All rights reserved.
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