Journal
MOLECULAR DIVERSITY
Volume 8, Issue 3, Pages 177-187Publisher
SPRINGER
DOI: 10.1023/B:MODI.0000036233.58271.25
Keywords
antibody binding; digital photolithography; epitope screening; miniaturization; parallel synthesis; peptide chip; peptide microarray; PGA; PGR chemistry; protein binding assay
Categories
Funding
- NIH [R44 GM067364, GM49957]
- R. A. Welch Foundation [E1027]
- Michigan Life Sciences Corridor Fund
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The technologies enabling the creation of large scale, miniaturized peptide or protein microarrays are emerging. The focuses of this review are the synthesis and applications of peptide and peptidomimetic microarrays, especially the light directed parallel synthesis of individually addressable high density peptide microarrays using a novel photogenerated reagent chemistry and digital photolithography (Gao et al., 1998, J. Am. Chem. Soc. 120, 12698; Pellois et al. 2002, Nat. Biotechnol. 20, 922). Concepts related to the synthesis are discussed, such as the reactions of photogenerated acids in the deprotection step of peptide synthesis or oligonucleotide synthesis, and the applications of high density peptide chips in antibody binding assays are discussed. Peptide chips provide versatile tools for probing antigen-antibody, protein-protein, peptide-ligand interactions and are basic components for miniaturization, automation, and system integration in research and clinical diagnosis applications.
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