Journal
JOURNAL OF STRUCTURAL BIOLOGY
Volume 147, Issue 3, Pages 283-290Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jsb.2004.02.008
Keywords
cryo-electron microscopy; reference-based alignment; supervised classification; translation; translocation
Funding
- NCRR NIH HHS [P41 RR0121919] Funding Source: Medline
- NIGMS NIH HHS [R01 GM070768, R37 GM29169] Funding Source: Medline
Ask authors/readers for more resources
A method of supervised classification using two available structure templates was applied to investigate the possible heterogeneity existing in a large cryo-EM dataset of an Escherichia coli 70S ribosome-EF-G complex. Two subpopulations showing the ribosome in distinct conformational states, related by a ratchet-like rotation of the 30S subunit with respect to the 50S subunit, were extracted from the original dataset. The possible presence of additional intermediate states is discussed. (C) 2004 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available