Journal
ANTIVIRAL RESEARCH
Volume 63, Issue 3, Pages 209-215Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.antiviral.2004.05.001
Keywords
Lassa virus; SARS coronavirus; Ebola virus; real-time PCR; imidazole nucleoside analogues
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Funding
- NIAID NIH HHS [9RO1 AI55452, R01 AI055452] Funding Source: Medline
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This report describes the application of real-time PCR for testing antivirals against highly pathogenic viruses such as Lassa virus, SARS coronavirus and Ebola virus. The test combines classical cell culture with a quantitative real-time PCR read-out. The assay for Lassa virus was validated with ribavirin, which showed an IC50 of 9 mug/ml. Small-scale screening identified a class of imidazole nucleoside/nucleotide analogues with antiviral activity against Lassa virus. The analogues contained either dinitrile or diester groups at the imidazole 4,5-positions, and many of which possessed an acyclic sugar or sugar phosphonate moiety at the imidazole 1-position. The IC50 values of the most active compounds ranged from 5 to 21 mug/ml. The compounds also inhibited replication of SARS coronavirus and Ebola virus in analogous assays, although to a lesser extent than Lassa virus. (C) 2004 Elsevier B.V. All rights reserved.
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