Journal
PLANTA MEDICA
Volume 70, Issue 9, Pages 813-817Publisher
GEORG THIEME VERLAG KG
DOI: 10.1055/s-2004-827228
Keywords
sulfated polysaccharides; Herpes simplex virus type I (HSV-1); Chlorophyta (green algae); Ulvophyceae; antiviral activity
Ask authors/readers for more resources
In order to evaluate the potency of novel antiviral drugs, 11 natural sulfated polysaccharides (SPs) from 10 green algae (Enteromorpha compressa, Monostroma nitidum, Caulerpa brachypus, C. okamurai, C scapelliformis, Chaetomorpha crassa, C. spiralis, Codium adhaerens, C. fragille, and C. latum) and 4 synthetic sulfated xylans (SXs) prepared from the beta-(1,3)-xylan of C. brachypus, were assayed for anti-Herpes simplex virus type 1 (HSV-1) activity. Except for one from E. compressa, all SPs showed potent anti-HSV-1 activities with 50% inhibitory concentrations (IC50) of 0.38 - 8.5 mug/mL, while having low cytotoxicities with 50% inhibitory concentrations of >2900 mug/mL. Anti-HSV-1 activities of SXs were dependent on their degrees of sulfation. To delineate the drug-sensitive phase, 4 polysaccharides, which showed potent anti-HSV-1 activities, were applied to time-of-addition experiments. Among the polysaccharides tested, 3 polysaccharides (SX4, SP4 from C. brachypus, and SP11 from C. latum) showed strong anti-HSV-1 activities with IC50 of 6.0, 7.5, and 6.9 mug/mL, respectively, even when added to the medium 8 h post-infection. These experiments demonstrated that some sulfated polysaccharides not only inhibited the early stages of HSV-1 replication, such as virus binding to and penetration into host cells, but also interfered with late steps of virus replication. These results revealed that some sulfated polysaccharides from Teen algae should be promising candidates of antiviral agents which might act on different stages in the virus replication cycle.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available