Biodegradability of poly (2-hydroxyethyl methacrylate) in the presence of the J774.2 macrophage cell line

The degradation of cross-linked and linear poly(2-hydroxyethyl methacrylate) (pHEMA), was examined in vitro with J774.2 cells. pHEMA microbeads were prepared with both types of polymers. Only cells in contact with the microbeads increased their production of lysosomal enzymes (TRAcP and ANAE) and released large amounts of reactive oxygen species with both types of pHEMA microbeads. Electron microscopy showed that macrophages were able to erode the Surface of linear pHEMA but unable to erode the surface of the cross-linked polymer. Cells appeared wared wrapped by the linear pHEMA surface, but those cultured on the cross-linked polymer were only laying, at the Surface. After cell Culture, the surface roughness of pHEMA slices was observed by atomic force microscopy (AFM). There was a significant increase in roughness (R,,) of the surface of linear pHEMA slices cultured with J774.2 cells whereas no difference in R-a between the surface of cross-linked pHEMA slices could be measured. AFM image of the hydrated materials were done: the Surface of linear pHEMA swelled considerably in saline whereas the hydrated cross-linked polymer did not differ from the air-dried appearance. In conclusion, linear pHEMA swells in biological fluids, activates macrophages in close contact with the polymer and can be progressively eroded. (C) 2003 Elsevier Ltd. All rights reserved.