Journal
PLANT CELL REPORTS
Volume 23, Issue 3, Pages 155-158Publisher
SPRINGER
DOI: 10.1007/s00299-004-0816-9
Keywords
phytosulfokine; peptide; transformation; nurse culture; antibiotics
Categories
Ask authors/readers for more resources
The relative growth rate of plant cells in vitro is considerably affected by initial cell density. This troublesome effect has interfered with the establishment of efficient plant cell culture systems, especially when only a small number of cells are expected to survive, such as in the genetic transformation of cells under antibiotic selection. To improve the recovery of antibiotic-resistant cells, we examined the use of the peptide plant hormone phytosulfokine (PSK), which has been shown to promote cellular growth and development in vitro. The addition of PSK to selective media increased the recovery of transformed callus from Agrobacterium-infected carrot hypocotyl explants from 7% to 39%, which is more than a fivefold improvement over the control. Most calluses developed into normal plantlets with cotyledons and primary roots and, eventually, formed foliage leaves. Thus, chemical nursing using PSK shows promise as a tool for basic research in plant biology and biotechnological applications.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available